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Since the MG conformation is more flexible than a fully folded polypeptide, this could lead to better packing at the dimer interface, leading to more stable dimers
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Samples were incubated at 25°C for 10 min
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In addition, the extent of aggregation as measured by elastic light scattering (Fig
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Target enzyme recognition by calmodulin: 2
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6% w/w BLG solution in 30 mM NaCl was 7
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ThT binds to oligomers and shows large amplitude in the slow second phase in response to formation of spherical aggregates and protofibrils, well in accordance with its reported amyloid specificity (Naiki et al
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As a result, disks containing larger proteins have lower unfavorable entropic contribution TΔS* to their kinetic stability ΔG* = ΔH*-TΔS*
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, in the Journal of Nutrition ("The Emerging Role of Dairy Proteins and Bioactive Peptides in Nutrition and Health," vol
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The difference in configurational entropy between two molecular conformations a and b can be estimated as ΔS = k^sub B^/2 ln(detσ^sub a^/detσ^sub b^), where σ^sub a^ and σ^sub b^, are the covariance matrices of atomic positional fluctuations of the two conformers and k^sub B^ is Boltzmann's constant
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DISCUSSIONComparison of spFRET histograms and ensemble trFRET measurementsOur spFRET results for the wild-type ribozymes show the presence of extended (undocked) and docked conformations, with relative populations that are strongly dependent on the Mg^sup 2+^ concentration
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The streptavidin biotin-immunoperoxidase method was used for the immunolocalisation of collagen types I and II
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At each timepoint three replicate samples were analysed providing a total of nine replicate values from three separate experiments
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Our data record total beneficiary-paid expenditure but do not record the number of beneficiaries who elected to pay
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