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CD measurements suggested that unfolding of GR was complete at 7 M GdnHCl, a result that was consistent with the iodide quenching data
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MATERIALS AND METHODS Materials Horse apomyoglobin was prepared by using Hapner's method (Hapner et al
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A similar principle underlies the design of energy landscape theories for folding kinetics that are based on Go models (105-107)
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The average topology in the EV limit is independent of amino acid sequence
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Three pHs were chosen based on the steady-state fluorescence results: pH 5, close to the midpoint of the titration observed in ANS-binding and fluorescence intrinsic experiments; neutral pH, where the intensity of steady-state fluorescence experiments was at its maximum; and pH 12, in the basic region
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Crystal structure of human butyryl cholinesterase and of its complexes with substrate and products
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Two ml of a buffered BLG solution was dialyzed against buffered PEG solutions of known osmotic pressure (15,16) at 20°C for 24 h
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ThT binds to oligomers and shows large amplitude in the slow second phase in response to formation of spherical aggregates and protofibrils, well in accordance with its reported amyloid specificity (Naiki et al
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There are four known families of PLCβ enzymes and all are activated by Gα^sub q^-subunits (for review, see Rebecchi and Pentyala, 2000; Rhee, 2001)
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Such protofilaments merge and intertwist, yielding thin fibrils, which are capable of further association and twining, producing mature amyloid
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Functional and immunological characterization of human-immunodeficiency-virus type-1 envelope glycoproteins containing deletions of the major variable regions
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The objectives of the research [14] reported in this paper are to produce toners using binders with high flexibility (low T^sub g^ and high elongation) and to evaluate the properties of fabrics printed with these toners
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Parturition occurred between mid-January and mid-March, and peaked in February
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The method was also successful with 102 eggs collected from Wakasa Bay (Table 4), which had been immediately fixed with 5% formaldehyde in seawater
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(5) Longs had a 53-week fiscal year in 2001
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It was Schering-Plough that ultimately brought Claritin over the counter as its patent expiration neared, but not until the company had exhausted every avenue trying to extend the duration of its monopoly
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The limitations of the study need to be considered before generalizing the results obtained
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They are being promoted to management positions that were not available to them in the past
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Instead, NFU folds and concedes a $1,200 pot
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In 2004 alone, IIX-21 conducted nearly twice the average number of flight and ground test hours as in the previous twenty years
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Because of these limitations, the probabilistic models may not be general enough to ascribe odds and explain play
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