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Since K^sub SV^ equals the product of the bimolecular quenching rate constant (k^sub q^) and the excited-state lifetime of the fluorophore ([tau]), the value of K^sub SV^ obtained from steady-state fluorescence measurements is dependent on the average lifetime allegra medicine .
The first regime 0 ≤ q 0 allegra medicine .
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In particular differences in the extent of protein aggregation, in structural compactness, or simply in the structural arrangement of tryptophan residues in unfolded states can change the magnitude of the red shift observed during the unfolding transition significantly allegra medicine .
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In addition, using the simple general equation, which covers for both fast and slow exchange, derived by Ishima and Torchia (34), we also show that the exchange rate can be determined from a combination of CPMG measured R^sub ex^ and change in R^sub ex^, (ΔR^sub ex^, vide infra) without prior assumption on the exchange timescale allegra medicine .
The results help to elucidate the role of apolipoprotein size and hydrophobicity for HDL stability, to uncouple apolipoprotein unfolding, dissociation, and lipoprotein fusion, and thereby gain a better insight into molecular determinants for the structural stability and fusion of HDL disks and spheres allegra medicine .
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