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Since no crystal structure of ACYI is available, all four of the components (A and S, I, II, and III) of Trp fluorescence defined previously (33,34) were included in the curve-fitting process
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Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy
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Fluorescence measurements in ice were conducted as previously described (12)
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Although suitable data are not available to establish if there is a direct correlation between the above ANS binding capacity and the actual instability to freeze-thawing (depending on the protein, irreversible damage has been estimated by diverse criteria such as loss of enzymatic activity, degree of aggregation, and loss of secondary or tertiary structure and, further, the outcome is generally influenced by the rates of freezing and thawing), it is a common observation that multimeric enzymes tend to be less stable to freezing than their monomeric counterpart (17)
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Furthermore, the localized spectra revealed different uptake kinetics of an externally applied drug into the individual subcellular compartments
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At pHs in the vicinity of the isoelectric point (pH 5
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Error analysis of configurational entropiesThe configurational entropy differences between complex and free forms reported above were obtained from the entropy differences at 8 ns (the full trajectory length used for analysis)
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The intensity of positive immunostaining was generally moderate but was not graded
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This study represents the first independent audit of the numbers, and viability, of the cells which are intended for implantation into cartilage defects of patients
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Macao, a special administrative region of China governed underthe policy of ''one country, two systems'' similar to Hong Kong,though only one-50th the size of its neighbor, liberalized thegambling industry in a bid to revitalize the sluggish economy byluring foreign investment
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Richard Canfield, an art lover and bibliophile, operated this establishment during New York's Gilded Age
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