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. Interestingly, no significant spectral changes were detected up to 1 M GdnHCl, whereas an increase in reactivity of one thiol group of GR to DTNB was observed at this concentration of GdnHCl (Fig
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. Hydration of apomyoglobin in native, molten globule, and unfolded states by using microwave dielectric spectroscopyKamei, TakashiABSTRACT The high resolution dielectric spectra of semidilute solutions of apomyoglobin in native (N, pH = 5), acid-- induced molten globule (A, pH = 4), and unfolded (U^sub A^, pH = 3) states have been measured in the range from 0
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. MATERIALS AND METHODSPeptides and proteinsThe synthetic peplide corresponding to residues 86-105 of Gβ^sub 1^ (NH^sub 2^-TTNKVHAIPLRSSWVMTCAY-CONH^sub 2^) and the corresponding control peptide that does not activate PLCβ (Gβ86-105(M101N)) were gifts from Dr
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. Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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. semifasciata larvae and posttransition juveniles indicate the presence of at least three main reproductive grounds, one located off Peninsula Valdes (42-43[degrees]S, 63[degrees]W), another off the coast between Engano Bay and Isla Escondida (43-44[degrees]S, 64[degrees]W to the coast), and the third off northeastern Camarones Bay (44-45[degrees]S, 65[degrees]W to the coast)
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