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. 1 B), resulted in the full exposure of one SH group per subunit
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. In addition, the distance distributions are peaked at larger distance values as sequence separation increases
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. the intensity of the fluorescence spectra decreased (Fig
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. Although a quantitative relation between aggregation and reduction of the unfolding induced red shifts cannot be given, our data indicate that protein aggregation contributes significantly to smaller magnitudes of red shifts as observed during thermal unfolding
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. The activity of the inhibited batch was Wild-type DmAChE was expressed in the baculovirus system (38)
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. To probe the origin of the two kinetic phases, we recorded Θ^sub 222^(t) data from the same apoA-2:DMPC sample in repetitive T-jumps
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. In addition, slower multiphase unfolding is detected at higher denaturant concentrations
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. DISCUSSION AND CONCLUSIONSWe have discussed a conceptual approach to the protein landscape problem that attempts to remain faithful to the high dimensionality of the system
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