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. Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig
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. 3 A) consisted of several overlapped components, which could be successfully distinguished by resolution-enhancing techniques, FSD (Fig
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. A scan of the protein structure database has shown that >75% of the proteins are glycosylated in nature
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. Of particular interest is the value of R^sub o^, which is related to the persistence length and also provides a measure of the goodness of the solvent because R^sup 3^^sub o^ quantifies the average volume per residue set aside by the chain for interactions with the surrounding solvent (38,41)
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. For reasons to be discussed below, this assertion is in fact inaccurate
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. Similar data were obtained for all protein sequences shown in Table 1
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. Thermodynamics of dcnaturant-induced unfolding of a protein that exhibits variable two-state denaturation
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. 1°C/min reveals for all protein concentrations λ^sub max^-values around 345 nm for the unfolded state
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. The study was supported by the European Union program under contracts No
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. 1 illustrates DNA compaction in PEG 3000 with an increase in the NaCl concentration
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. Third, in contrast to the reversible unfolding of lipid-free protein (indicated by the agreement between the heating and cooling CD curves in Fig
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. The V4 loop (residues 398-409), for which electron density is missing in all crystal structures, and the V3 loop, which has been proposed to undergo significant rearrangements upon CD4 binding, were modeled onto the gp120 core, together with the six core mutations of the LAI strain (98
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. Quenching experiments In experiments aimed to detect protein self-association, the concentration of EI^sup sc^ was varied at pH 7 from 0
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