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. More importantly, the position of the negative peak, which reflects the unfolding of the native structures, was centered at around 1628 cm^sup -1^ in the temperature range 30-66°C, then shifted to around 1639 cm^sup -1^ when the temperature increased from 66°C to 80°C, and finally remained at this wavenumber upon heating
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. Spectra from the relaxation-compensated CPMG experiments were integrated and analyzed in the same manner as the R^sub 1^ and R^sub 2^ experiments
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. We have previously found that activated PLCβ-Gβγ complex can be rapidly deactivated by Gα(GDP) subunits without dissociation, which led to the suggestion that Gα(GDP) binds to PLCβ-Gβγ and perturbs the activating interaction without significantly affecting the PLCβ-Gβγ binding energy
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