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. These results indicated the presence of an expanded predissociated dimer at 2
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. These changes led to burial of the Tip and/or Tyr and other regions susceptible to trypsin digestion
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. and CTQ2004-04474 and CTQ2005-00360/ BQL' from MEC
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. The same figure also shows the S^sup 2^^sub f^ contours when an additional nanosecond-timescale internal motion is present with time constant τ^sub is^ = 2 ns and order parameter S^sup 2^^sub s^ = 0
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. The liquid-liquid coexistence region and glass transition are well separated, and so the two processes will not affect one another
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. Previous work by us and others have employed protein engineering and cysteine labeling to attach specific probes to proteins to record conformational changes (Hammarström et al
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. The physicochemical reason for ThT fluorescence in the presence of amyloid fibrils is not known (LeVine, 1993)
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. Hydrostatic pressure has been used extensively in the past to measure the volume change of protein-protein associations in solution, but it has not yet been used to monitor associations of membrane-bound proteins
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. Denaturation kinetics of apoA-2:DMPC disksTo correlate the heat-induced lipoprotein fusion with the protein unfolding and to probe the kinetics of these transitions, we recorded CD and light-scattering data at 222 nm upon sample heating and cooling at a rate from 1
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. In detail the folding energy landscape will look more like a series of low-dimensional terraces (inset to Fig
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. 3 A shows fluorescence signals from freely diffusing ribozyme molecules labeled with Cy3 (donor) and Cy5 (acceptor) at 0
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. The +4°C shift, the reduced height, and the increased width (decreased cooperativity) of this peak as compared to the sharp transition in DMPC vesicles at 24°C (Chapman, 1975) are characteristic of lipoprotein disks that have smaller co-operative lipid domains than vesicles
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. Ohia is one of a few Blacks to head schools of pharmacy in the nation
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