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. The optimal temperature for ACYI activity is ~60°C (25,26), which indicates that it can be regarded as a thermostable enzyme
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. The unfolding of this β-sheet allows the water molecules to penetrate into the interior of the catalytic domain, which results in the transformation of some Class I fluorophores to Class II and a further inactivation of the enzyme
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., 1993) and rabbit muscle creatine kinase (Couthon et al
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. Molecular Cell Biology, 4th ed
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. Bis-ANS has a shorter lifetime than ANS but also displays two different components with a more even distribution
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. The resulting Θ^sub 222^(t) data, which showed CD changes during the dead-time of the experiment (Differential scanning calorimetryAn upgraded MC-2 microcalorimeter (Microcal, Amherst, MA) was used to record excess heat capacity of the apoA-2:DMPC complexes
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