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The equilibrium unfolding of GR by GdnHCl was monitored by measuring fluorescence emission spectra at various denaturant concentrations clomid cost does much .
Nevertheless, the 2D IR analysis also suggested that the pretransitional conformational changes involved the unfolding of the solvent-exposed β-sheet structures and aggregates appearing at a temperature at which the protein had not fully unfolded clomid cost does much .
1-cm path length cuvette on a Jasco spectropolarimeter 715 (Jasco, Tokyo, Japan) attached to a Peltier PTC-348 WI clomid cost does much .
Similarly, only 13 out of 53 hydrophobic interactions are intrasubunit in nature clomid cost does much .
Denaturant m-values and heat capacity changes: relation to changes in accessible surface areas of protein unfolding clomid cost does much .
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Interpreting the folding kinetics of helical proteins clomid cost does much .
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In addition, kinetic information on subpopulations interconverting on the microsecond to millisecond timescales can be obtained from a detailed analysis of the FRET histogram peak shapes clomid cost does much .
For the purpose of this article, we have used a value of 1 for the correction factor [gamma], which is a ratio of the products of fluorescence quantum yield and the instrument detection efficiency in the two channels clomid cost does much .
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