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As previously pointed out (Rietveld and Ferreira, 1996; 1998; Ferreira and De Felice, 2001), dimerization may lead to a significant increase in stability against chemical modification of GR, including thiol oxidation, de-amidation, or proteolytic digestion
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Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins
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Role of osmolytes as chemical chaperones during the refolding of aminoacylase
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Two-dimensional infrared correlation spectroscopy study of the aggregation of cylochrome c in the presence of dimyristoylphosphatidylglycerol
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5 mM PEP at pH 7
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The spectra were recorded in a 0
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It is widely accepted that pico- to nanosecond thermal fluctuations serve as a lubricant for functionally relevant motions occurring on a micro- to millisecond timescale (6)
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They are active as dimers, where the two basic regions bind DNA symmetrically as α-helices on opposite sides in the major groove
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Nuclei that are influenced by chemical exchange have been shown to correlate with residues known to be critical for protein interactions and enzymatic activity (39,40, 41,42,43,44)
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47 ml g^sup -1^, obtained from the R^sub h^ of the BLG monomer in dilute solution, leads to values of φ for the more concentrated solutions which are above the expected φ^sub max^ or, using the reverse of the above conversion, a mass fraction of 43
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The plateau level is preserved for DCVJ over 300 h, showing that this probe also binds to larger assemblies (spherical aggregates and protofibrils)
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We are grateful to Per Persson for initial assistance with electron microscopy work and to Jeffery Kelly and David Wemmer for providing the pmmHα plasmid
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Although amyloidal isolates either from in vivo samples or in vitro preparations are generally straight, some of them display more or less pronounced curvature (e
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