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. 07, and are fairly uniform throughout the sequence, except for five outliers with S^sup 2^^sub s^ values between 0
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. Fluorescence anisotropy data from Bis-ANS bound to the different unfolded and aggregated states showed modest anisotropy values (compared to when bound to the thyroxine binding site in the native TTR tetramer), revealing that Bis-ANS docs not penetrate the protein but is more loosely associated to the protein surface (data not shown)
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