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. Interestingly, no significant spectral changes were detected up to 1 M GdnHCl, whereas an increase in reactivity of one thiol group of GR to DTNB was observed at this concentration of GdnHCl (Fig
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. Proteins can adopt totally different folded conformations
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.The influence of small differences in primary and quaternary structure and in degree of glycosylationDifferences in primary and quaternary structure, degree of glycosylation, and oligomeric state, between DmAChE and HuBChE, although they affect various structural and functional parameters, are not reflected in changes in global dynamics on an Ångström-nanosecond scale in the temperature range investigated
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. At the lower concentrations, Newtonian behavior was observed at shear rates, γ, in the range 10-100 s^sup -1^
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. The fluorescence intensity of Bis-ANS decreases as the A-state aggregates, well in accordance with hiding of accessible surface area in contrast to the behavior of ANS, likely due to the fact that Bis-ANS is more bulky
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.Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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