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. One of the best ways to address this kind of puzzle is to look for instances of pleiotropy, a known and common phenomenon of genetics
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. An additional step of affinity purification was done using a lactosyl-Biogel column to ensure the presence of only the active, refolded molecules in the rSBA sample finally obtained (21)
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. This latter observation has been ascribed elsewhere to contributions to the scattering signal from the hydration water protons, which are more flexible than protein protons in the temperature range above 260 K (44)
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. The environmentally sensitive probe ANS has previously proven useful in detecting molten globule states, starting with the work by Semisotnov and co-workers (Semisotnov et al
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. The α-helix content was estimated from the value of [Θ] at 222 nm (Mao and Wallace, 1984)
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. 1 B and by their independence of the scan rate), the heating and cooling curves of the apoA-2:DMPC complexes show hysteresis that was also observed in DMPC complexes with apoC-1 (Gursky et al
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. Thermally Induced Phase SeparationThermally induced phase separations such as liquid-liquid separation (LLPS) and crystallization in polymer solution are determined by thermodynamic equilibrium and the kinetics of the process
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. Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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