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. Two intermediate states were populated at 2-3 M GdnHCl, as indicated by a combination of spectroscopic and chromatographic methods
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. This indicates that subtle conformational changes are induced by low concentrations of GdnHCl (The existence of partially folded intermediate states in the unfolding of GR is indicated by a comparison of fluorescence spectral shift, anisotropy, iodide quenching, and CD data as a function of GdnHCl concentration
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