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Two intermediate states were populated at 2-3 M GdnHCl, as indicated by a combination of spectroscopic and chromatographic methods diazepam clonazepam .
This latter observation has been ascribed elsewhere to contributions to the scattering signal from the hydration water protons, which are more flexible than protein protons in the temperature range above 260 K (44) diazepam clonazepam .
The A-state is of molten globule type and shows a dramatic increase in β-sheet content evident from a spectral peak at 213 nm (Fig diazepam clonazepam .
Fluorescence characterization of different protein conformersThe A-state of TTR binds the classic hydrophobic dye ANS (Lai et al diazepam clonazepam .
RELATIONSHIP TO OTHER MODELSWe stress that the search-space picture explored above is not incompatible with the frameworks or results of the other models discussed in the introduction, but rather serves to show how the apparently alternative models are related, and how high-dimensional features are implicit in all of them diazepam clonazepam .
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These studies have provided thermodynamic information on the global folding of the hairpin ribozyme (Klostermeier and Millar, 2000) and also quantified the energetic contributions of individual tertiary interactions (Klostermeier and Millar, 2001a, 2002) diazepam clonazepam .
The resultant frequent excursions to this quasidocked structure can facilitate subsequent formation of specific contacts between the two loops, thereby stabilizing the resulting tertiary structure by reducing the entropie penalty for docking diazepam clonazepam .
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