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DISCUSSIONMolten globule (MG) folding intermediates have been characterized for a number of proteins (for reviews, see Kuwajima, 1989; Ptitsyn, 1992, 1995)
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It is generally assumed that two distinct enzymes are involved in the intracellular degradation of N-acetylated proteins: the N-acetylated peptide hydrolase catalyzes the hydrolysis of the N-terminal amino acid residues from the N-acetylated proteins causing the release of acetylamino acids, which are finally hydrolyzed by ACYI (10,11)
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The protein concentration used was 0
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This conclusion has been reached through quantitative studies of the scaling of hydrodynamic radii (46) and radii of gyration (11,47) with chain length under harshly denaturing conditions
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1 C) and the FTIR data acquired at pH 3 (Table 2)
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As a consequence the corresponding emission spectra that were measured during unfolding appear completely different (Fig
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Wassink, Soldering in Electronics (Ayr, Scotland: Electrochemical Publications, 1984), pp
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The physicochemical reason for ThT fluorescence in the presence of amyloid fibrils is not known (LeVine, 1993)
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Furthermore, the evolutionary tailoring of nonnative interactions provides additional "design-space" within which a pathway to the folded state may be coded, but without compromising the stability of the final, native state
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As in the case of the Φ-value analysis, this experiment is also usually interpreted as giving information about the transition state (Oliveberg et al
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