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. Phase diagram for acidic conformational states of apomyoglobin
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. For small n, we obtain softer repulsive potentials
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. 1°C/min) gives rise to less pronounced aggregation during the thermal unfolding transition as compared to higher heating rates; and (2), the lowest protein concentration (7 µg/ml) reveals a less-pronounced aggregation also for a higher heating rate (1°C/min), whereas higher protein concentrations studied with the higher heating rate exhibit more pronounced aggregation
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. Sample preparation Before sample preparation, all protein stocks were dialyzed against 2 mM Hepes buffer, pH 7
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. 7, with background corrected from unbound probe
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. Contact order, transition state placement and the refolding rates of single domain proteins
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. brasilianus was far less abundant than P
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. This truncated form of gp120 (core gp120) does not compromise much the biophysical and biological properties compared to the fulllength wild-type (Kwong et al
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. The ribozyme construct contains the naturally occurring 4WJ (Fig
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. 1 min for the wild-type (WT) 2WJ, 3WJ, and 4WJ ribozymes, respectively
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. More importantly, the position of the negative peak, which reflects the unfolding of the native structures, was centered at around 1628 cm^sup -1^ in the temperature range 30-66°C, then shifted to around 1639 cm^sup -1^ when the temperature increased from 66°C to 80°C, and finally remained at this wavenumber upon heating
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