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Titration of reactive thiol groupsThis was performed by following the increase in 2-nitro-5-thiobenzoic acid (TNB) absorbance at 412 nm, according to Collier (1973)
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The decrease in the content of Class I Trp fluorophores suggested that the intermediate had a loose tertiary structure, which allowed the penetration of water molecules into the hydrophohic interior
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It may also be possible that a smaller degree of subunit dissociation in part explains the sharp increase in the residual enzymatic activity, retained after freeze-thawing, with increasing protein concentration (14,16,29)
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Excitation was achieved by focusing the 514-nm line of a 543-AP-A01 tunable argon-ion laser (Melles Griot, Carlsbad, CA) inside the sample solution, 10 µm from the glass coverslip surface, using a Plan Apo 100 ×, 1
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Patients paid nothing pre-RP but paid about $0
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CompetitivenessAn independent samples t-test indicated that men (M = 61
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Evidence of this deeper emotional involvement is indicated in the SOGS scores of the current study's seven participants whose scores indicate possible or probable gambling problems (e
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