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INTRODUCTION Posttranslational modification is one of those events in biology that not only adorn a protein with variety but also impart it with different physicochemical properties ionamin site sys.kcn.ru .
This feature is shared with Gaussian chains (39), although clear differences exist in the magnitude of and correlation between conformational fluctuations ionamin site sys.kcn.ru .
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The experimental results indicate that at physiological pH, the dimeric EI^sup SC^ had a well-folded structure; however, at low pH, EI^sup SC^ showed a partially unfolded state with the features of a molten globule, as suggested by fluorescence, far-ultraviolet circular dichroism, FTIR, and 8-anilino-1-naphthalene-sulfonic acid binding ionamin site sys.kcn.ru .
Cholinesterases belong to the α/β hydrolase fold family (19) ionamin site sys.kcn.ru .
Here we report a study of the backbone dynamics by ^sup 15^N-spin relaxation on the CaM dimer in complex with a dimeric peptide that mimics the CaM binding region of the bHLH transcription factor SEF2-1 ionamin site sys.kcn.ru .
We find that the association of Gβ86-105 to PLCβ gives a volume change that is similar to the full Gβγ-subunit ionamin site sys.kcn.ru .
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of Physiology and Biophysics, Boston University School of Medicine, W329, 715 Albany St ionamin site sys.kcn.ru .
CONSEQUENCES OF HIGH-DIMENSIONAL SEARCHESWe have identified two general predictions of this high-dimensional view of folding: 1), the sequential diffusive exploration of low-dimensional subspaces favored by fast folding; and 2), the suggestive stabilization of these subspaces by discriminate but nonnative (or near-native) interactions, without recourse to long-range guiding forces ionamin site sys.kcn.ru .
RELATIONSHIP TO OTHER MODELSWe stress that the search-space picture explored above is not incompatible with the frameworks or results of the other models discussed in the introduction, but rather serves to show how the apparently alternative models are related, and how high-dimensional features are implicit in all of them ionamin site sys.kcn.ru .
We also thank Atila Gosztonyi, Raul Gonzalez, and two anonymous reviewers for providing useful comments on the manuscript ionamin site sys.kcn.ru .
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747ErrorRelative fecundity (basedon prefertilization oocytes)Equality of slopes0 ionamin site sys.kcn.ru .
RNA folding: models and perspectives ionamin site sys.kcn.ru .
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