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. Powders were dissolved in buffer (for details see below) and all enzymes were purified by the use of a desalting column (PD-10, Sephadex G-25, Amersham Biosciences, Freiburg, Germany)
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. However, Tara et al
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. Cytoplasmic concentrations relevant to macromolecular crowding are typically up to 40% v/v (72)
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. The complete reaction can, for simplicity, be divided into four phases, which are schematically depicted in Fig
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. 39(1) Absolute fecundity for each age group is the estimated fecundity(based on fertilized eggs) for ages 7, 10, 13, and 16, respectively
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. In order to sustain these species it is especially important that their distribution and biomass at all life stages are known
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. major were obtained from adult female fish that had spawned in isolation tanks at several sea farming centers described in Table 2
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. The quasi-docked conformation interconverts rapidly with an extended structure, giving rise to continuous peak shifts with added Mg^sup 2+^, as well as pronounced asymmetry and broadening in the FRET efficiency histogram at high Mg^sup 2+^ concentrations
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. 1 M sodium cacodylate buffer at pH 7
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