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. Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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. An estimation of the T^sub m^ of EIC^sup sc^, based on the fluorescence results (Fig
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. Furthermore it was demonstrated here in the case of homologous α-amylases, that accompanying studies with additional techniques (CD, static, and dynamic light scattering, see for example (14,36) for analyzing the compactness in terms of radii of gyration of unfolded states) can help to obtain a more meaningful interpretation of the tryptophan fluorescence data
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. Pressure- and heat-induced inactivation of butyrylcholinesterase: evidence for multiple intermediates and the remnant inactivation process
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. Pressure has profound effects on the highly compressible membrane interior promoting phase transition to gel phases and, for the studies presented here, the phase transition will occur ~1
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. This is of course a restatement of Levinthal's paradox (Levinthal, 1969; Karplus, 1997), but a helpful one, in that the two necessary assumptions for the paradox to arise are clearly seen
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. The formation of the globule itself from the denatured state corresponds in this picture to the first hypergutter in a series
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. Column calibration was done with a set of eight proteins of known molecular weights (thyroglobulin, 669 kDa; ferritin; 440 kDa; alcohol dehydrogenase, 150 kDa; bovine serum albumin, 66 kDa; ovalbumin, 45 kDa; carbonic anhydrase, 29 kDa; trypsinogen, 24 kDa; and cytochrome C, 12 kDa)
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.Ralf Jansen,* Wojciech Dzwolak,[dagger] and Roland Winter** University of Dortmund, Department of Chemistry, Dortmund, Germany; and [dagger] High Pressure Research Center, Polish Academy of Sciences, Warsaw, PolandSubmitted July 1, 2004, and accepted for publication November 15, 2004
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. The broad asymmetric peak shape observed for the SL 4WJ implies that extended undocked and quasi-docked states are in rapid conformational exchange, and our kinetic simulations indicate that the rate constant for formation of the quasi-docked state is in the range 10-41 × 10^sup 3^ s^sup -1^, whereas the corresponding reverse rate constant is in the range 3
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