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The phase diagram analysis, which is a sensitive tool to detect folding intermediates (35), was performed to characterize whether an intermediate was involved in ACYI thermal unfolding
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INTRODUCTIONProtein dynamics and function occur on timescales spanning several decades, from femtosecond electronic rearrangements (e
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The CD melting curves at 222 nm, Θ^sub 222^(T), recorded during sample heating and cooling from 1 to 80°C, show that the helical structure reaches maximum at 25°C and completely unfolds upon heating as well as cooling from 25°C (Fig
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t] = 1] - E[[CostDayNSD
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.TheoryThe equations for the relaxation of a backbone amide ^sup 15^N spin in a protein as well as their interpretation in terms of the model-free approach of Lipari and Szabo (25) are well described in the literature (see, e
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