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A further aspect of nonequilibrium behavior exhibited by solutions of proteins with short-ranged attractive interactions is metastable liquid-liquid separation (11,12)
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Data fitting suggests that, in contrast to the double-exponential kinetics in the first jump (τ^sub fast^ = 190 s, τ^sub slow^ = 1630 s), the data in the second jump are well approximated by a single exponent with a relaxation time τ = 190 s, which is similar to τ^sub fast^
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Currently, we are unable to unambiguously rationalize the difference between the behavior of the immobilized 4WJ ribozyme and the 4WJ construct examined in our solution-based spFRET experiments
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