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The equilibrium unfolding of GR by GdnHCl was monitored by measuring fluorescence emission spectra at various denaturant concentrations
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Changes in secondary structure of GR induced by GdnHCl were examined by CD measurements (Fig
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In Protein Structure: A Practical Approach
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Mouse acetylcholinesterase unliganded and in complex with huperzine A: a comparison of molecular dynamics simulations
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For instance, the barnase (45) and oxalocrotonate tautomerase (46) bind specifically to their rigid target to form a specific complex with essentially one overall conformation
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Osmotic stress and nonequilibrium phase diagramIn Figs
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Studies on the solubility of lactoglobulin
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Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments
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An advantage of this PLCβ construct is that it can be bacterially expressed (see Wang et al
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However, not only do we find that pressure destabilizes the membrane binding of these G-protein subunits but that identical pressure behavior is seen for Gα^sub s^ even when its palmitoyl chains are removed by reducing agents
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Structural polymorphism of mature fibrilsFig
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However, a quantitative relation between the topomer search space and contact order is still unclear, since no native contacts are actually required to form at a purely topologically-defined transition state at all (although many are to be expected from the partial ordering at the secondary level at least)
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Recently fertilized eggs were approximately 850 [mu] in diameter
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Taken together, the smooth E shifts with changing Mg^sup 2+^ concentration combined with the observed broadening/ tailing at high Mg^sup 2+^ concentration are consistent with the presence of a dynamic equilibrium between the quasi-docked state and an extended undocked state, with a relatively small energy barrier for interconversion (Fig
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Because Basic is not a personalized strategy, it seems less likely to be maintained in the face of losses
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Because the computer made appropriate strategies effortlessly available, departures from Basic could not reflect ignorance or fatigue
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5%A/R (3), Eq (3), Child Care Learning FF (3), Inv (3), Centers220FasTracKids Int'l
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