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- "We're not a church that is about to go bankrupt," said the church's pastor, Andrew Mullins
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. Recently, the crystal structure of a D-aminoacylase from Alcaligenes faecalis was determined (16)
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. The other components remained at the same level as at 30°C in the temperature range of 50-66°C
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. 8 b shows the distribution of the iwo types of interactions
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. Similar data were obtained for all protein sequences shown in Table 1
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. Besides the disappearance of secondary structure elements, in most cases the three-dimensional structures are substantially altered
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. ANS binding experiments, extended to a set of proteins including serum albumin, α-amylase, β-galactosidase, alcohol dehydrogenase from horse liver, alcohol dehydrogenase from yeast, lactic dehydrogenase, and aldolase, confirmed that a stressed condition of the native fold in the frozen state appears to be general to most proteins and pointed out that oligomers tend to be more labile than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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. Hydrodynamic calculations on the CaM:SMLCK complex showed that its diffusion tensor is nearly axially symmetric with a (τ^sup o^^sub m^)^sub app^ pattern for the α-helices that is almost a mirror image of that in the CaM:SEF2-1mp complex, Fig
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. After dialysis the dialysis tube was removed from the PEG solution and the BLG concentration determined by spectrophotometry
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. 1 A, solid circles) indicates that, consistent with the earlier reports (Ritter and Scanu, 1979; Massey et al
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