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, 1995), and a single mutation is sufficient to prevent dimerization of human GR (Nordhoff et al, 1993) mechanism of action for metformin .
This is referred to as U' mechanism of action for metformin .
As for azurin (12), lowering the freezing temperature from -5°C to -13°C increases the number of binding sites but the average affinity is lowered mechanism of action for metformin .
However, repeating this study for solubilized proteins that were freshly dialyzed to remove detergent gave an apparent K^sub d^ only 10-fold weaker than predicted, suggesting that we may be not be viewing a simple protein-protein association (K^sub d^ ~ 70 nM; Fig mechanism of action for metformin .
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