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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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A local length scale spans five- to nine-residue stretches over which sequence-specific spatial correlations decay
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There are two obvious differences between our approaches
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These two sets of researchers use either coil library statistics (125) or conformations of residues in loops (114) to model local, sequence-context-dependent conformational preferences
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We do not expect there to be any major differences between EV-limit ensembles generated using our approach and the methods used by the Zhou and Sosnick groups
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3 M^sup -1^ and v = 0
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Notwithstanding these experimental results, we were not able to detect any differences in global MSDs between DmAChE and HuBChE, despite the former having a much lower degree of glycosylation than the latter
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6 Hz in the ^sup 1^H and ^sup 15^N dimension, respectively
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Fluorescence anisotropy data from Bis-ANS bound to the different unfolded and aggregated states showed modest anisotropy values (compared to when bound to the thyroxine binding site in the native TTR tetramer), revealing that Bis-ANS docs not penetrate the protein but is more loosely associated to the protein surface (data not shown)
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Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes
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1% Tween 20/PBS, and incubated with 4-chloro-1-naphthol solution (substrate of horseradish peroxidase in the kit) containing 0
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