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. At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range
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. Unfolding and refolding of porcine odorant binding protein in guanidinium hydrochloride: equilibrium studies at neutral pH
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. Water was doubly deionized and purified on a Millipore (Billerica, MA) system
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. It is known from various previous studies that for nearly all á-amylases both thermal and GndHCl-induced unfolding appear as an irreversible process (17-19)
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. Both lyophilized powders were placed in aluminum sample holders (30 × 40 × 0
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. Hence, there are four hinge regions in the CaM dimer
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. Taken together, our results suggest that these hydrocarbon chain modifications do not play a significant role in the membrane binding stability of these proteins in accord with previous results (Philip and Scarlata, 2004)
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. Alternately, it is possible that multiple Gβ86-105 peptides bind to the enzyme giving the same overall volume change
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. Furthermore, the amplitude of the light-scattering changes in apoA-1:DMPC complexes is significantly smaller than that in apoA-2 complexes, suggesting that apoA-1 inhibits disk fusion more effectively than apoA-2
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. Displacement of apo A-I from HDL by apo A-II or its C-terminal helix promotes the formation of pre-β^sub 1^ migrating particles and decreases LCAT activation
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. We also thank Atila Gosztonyi, Raul Gonzalez, and two anonymous reviewers for providing useful comments on the manuscript
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. When compared with the EIC^sup tt^
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. Instead, the broad linewidth in the vegetal cytoplasm may stem from magnetic inhomogeneity in that region
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. Large-amplitude thermal fluctuations, requiring adequate protein hydration, have been shown to be mandatory for the function of bacteriorhodopsin, the light-driven proton pump in the purple membrane of Halobacterium salinarum (7)
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. After lyophilization, the powder was dried over silica gel for two days and hydraled by H2O vapor exchange for two days over a saturated KNO^sub 3^ salt solution (93% relative humidity) at 25°C
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.Determination of atomic mean-square displacementsThe elastic intensity (Eq
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. We conclude that HuBChE stabilization by covalent modification with soman is due to an increase in the free energy of the unfolded state
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.CONCLUSIONSThe wettability assessment of Sn-2
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. The CaM:SEF2-1mp complex is found to be highly flexible with internal motions on the picosecond, nanosecond, and microsecond timescales
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. Irreversibility of the high-temperature differential scanning calorimetry (DSC) transition precluded its thermodynamic analysis
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.33 K/min is sigmoidal and reaches a plateau at high temperatures, suggesting that the unfolding transition is complete at 99°C, yet the light-scattering curve recorded in the same experiment does not reach a plateau, and the signal continues to increase upon cooling from 99°C (Fig
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. This study presents the multistage, hierarchical character of the spontaneous fibrillation of insulin at low pH and at 60 and 70°C, and-due to the marked enhancement of image resolution achieved-brings new clues as to the fibrils' ultrastructure and mechanisms of its assembly
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. 8 shows a comparison of the peak positions for the SL and WT versions of the ribozymes
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. Remarkably, the wild-type 3WJ can still form a catalytically active docked conformation, presumably by trapping a small population of molecules that transiently occupy a compact structure
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. Effect of transforming growth factor-beta 1 (TGF-beta 1) on matrix synthesis by monolayer cultures of rabbit articular chondrocytes during the dedifferentiation process
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.It is still not entirely clear how the misidentifications of Siddharth and Malvika occurred
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.--Pharmaceutical Science* MICHIGANUniversity of Michigan College of Pharmacy Ph
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