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Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig morphine addiction symptom .
The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph morphine addiction symptom .
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DNA compaction can be represented by a compaction curve as the dependence of the globule (compact DNA) fraction in solution on the concentration of the compaction agent, i morphine addiction symptom .
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