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. Since the dealkylated soman moiety bound to the HuBChE monomer subunit contributes only three hydrogen atoms (P-CH^sub 3^) to the enzyme-inhibitor conjugate, while the deglycosylated native enzyme monomer itself accounts for >3500 nonexchangeable hydrogen atoms, the contribution of the inhibitor is negligibly small
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. ANS increases its quantum yield in response to aggregate size, which is rather surprising given that more accessible surface area is provided in monomeric molten-globule states
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. The T-jump data to 95°C are well approximated by a single exponential, yet two exponents are required to fit the data at 75-90°C, suggesting two kinetic phases
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.The influence of inhibitor-binding on global enzyme molecular dynamicsAlthough several studies using different techniques have shown a modification of global protein molecular dynamics on an Ångström-nanosecond scale upon inhibitor-binding, we were not able to detect any significant differences between the native and the inhibited HuBChE, at comparable degrees of hydration, up to ~334 K (61°C)
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