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0 The scan rates were 30 K/h
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Before comparing the behavior of individual proteins it is convenient to divide the group into two classes: one (β-gal, α-amylase, and ALD), which binds ANS essentially only in the frozen state, and the rest, which bind a fair amount of ANS also in liquid solution (4,5,28)
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Lipid suppression has been especially important for selecting regions for localized spectroscopy, since at this high magnetic field strength, the lipid image of the lipid-rich oocyte significantly affects the water image, thus hampering correct voxel positioning
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The influence of inhibitor-binding on global enzyme molecular dynamicsAlthough several studies using different techniques have shown a modification of global protein molecular dynamics on an Ångström-nanosecond scale upon inhibitor-binding, we were not able to detect any significant differences between the native and the inhibited HuBChE, at comparable degrees of hydration, up to ~334 K (61°C)
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To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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Additionally, little change in membrane polarity and hydration of POPC/PI(4,5)P^sub 2^ (67:33) membranes are detected in this same pressure range (Rebecchi et al
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