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. Sharmistha Sinha* and Avadhesha Surolia*[dagger] * Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India; and [dagger] National Institute of Immunology, New Delhi, India Submitted July 3, 2006, and accepted for publication August 29, 2006
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. Reconstitution studies using the helical and carboxyl-terminal domains of enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system
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. Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach
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. As a result, a large number of Cu^sub 6^Sn^sub 5^ IMCs were found in the bulk of the Sn-Ag-Cu-Bi solder than in Sn-Pb solder (Fig
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. This is based on the observations that the bound SEF2-lmp lacks any well-defined conformation, and that not all hydrophobic peptide residues simultaneously interact with the hydrophobic patches on the inner surface of the CaM dimer (11)
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. The metastability is with respect to solid-liquid coexistence, that is, the solutions are supersaturated
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. TTR has been linked to a number of different amyloid diseases, including familial and sporadic ailments (Kelly, 1996)
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. For example, curvature in the "Chevron plot" that results from a plot of log k^sub f^ with [denaturant] is taken to indicate a change of transition state (and by implication the existence of an intermediate)
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. Glass transition temperature (T^sub g^) and melting temperature (T^sub m^) were determined by DSC (Seiko SII DSC 200C, Perkin Elmer Pyris I DSC)
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. Therefore, a purely diffusion-controlled on-rate k^sub on^ of 10 s^sup -1^ M^sup -1^ would put a lower limit on the CaM:SEF2-1mp affinity, k^sub off^/k^sub on^ > 10^sup -6^
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. All three probes bind with similar affinity to these oligomers with DCVJ, indicating the strongest affinity showing I^sub 50^ values of 1
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