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. There was no clear difference between N and A states in contrast to the 25% reduction of helix content and the 50% reduction of heat capacity change upon unfolding
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. In this work, we show that we can simulate conformational ensembles, with atomistic detail, such that ensemble characteristics match the predictions for polymers in the EV limit
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. Experiments that only detect close spatial contacts will be interpreted as providing evidence of long-range "residual" structure in the denatured state (94-98)
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. Of particular interest is the question of how preferential interactions in 8 M urea or 6 M GdnCl conspire to make these conditions mimic perfect solvents for proteins
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. Then, one is tempted to ask, is there any relationship among the different functions and structures of the PTS proteins on one side, and the thennodynamic parameters and folding properties on the other? To answer this question, it is necessary to describe the conformational preferences of proteins, where studies of different members of the family have been described
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. Steady-state ANS binding ANS binding was detected by collecting fluorescence spectra at different pH in the presence of 100 µM of dye
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. 4) of each sample at a given temperature T was normalized to its elastic intensity at the lowest temperature (~20 K) for each detector, and the values so obtained were plotted in a semilogarithmic plot versus Q^sup 2^
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. The effects of glycosylation, oligomeric state and differences in catalytic activity on molecular dynamics: AChE versus BChEGlycosylation appears to play a role in prolonging the circulatory lifetime of BChE and AChE (60,61), although it is not required for enzymatic activity
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. It was then suggested that PLCβ^sub 2^ binds to Gβγ-subunits through several strong contacts, but that activation proceeds through more subtle, weaker contacts
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. Fluorescence measurements and analysisFluorescence measurements were performed on an ISS spectrofluorometer (Champaign, IL)
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. , 1996) and the extension or stretching of the coiled-coil gp41, leading to the insertion of the membrane fusion peptide into the host membrane, which induces fusion of the viral and host membranes (Chan and Kim, 1998)
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. EI^sup sc^ was dephosphorylated by overnight dialysis at 278 K against phosphate potassium buffer (10 mM, pH 7
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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