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The distributions of distances are sharply peaked for near-neighbor residues and they become increasingly broad as sequence separation increases oxycodone 5 .
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In EI^sup ec^, the thermal midpoint of the transition monitored by fluorescence is concentration-dependent (52), but the slopes of the native baseline are smaller due to the fact that two tryptophan residues are observed in the C-terminus of EI^sup ec^ oxycodone 5 .
Unlike simple hyperbolic binding curves, profiles such as those of Fig oxycodone 5 .
This process may ensue in widespread deformations of the native fold with the creation of a large number of ANS binding pockets oxycodone 5 .
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, 1997, 1999, 2001a,b; Svensson et al oxycodone 5 .
Although the fluorescence lifetime of ANS is not in the range to enable accurate determination of the rotational correlation time of a large oligomer, ANS anisotropy decay can be used as a measure of aggregation both through point measurements and in a kinetic assay showing limiting anisotropy baselines oxycodone 5 .
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, 1998), as well as the four-way helical junction from which it is derived (F oxycodone 5 .
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