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. Fluorescence experiments at pH 7 and 9 showed a sigmoidal transition with T^sub m^ = 311 ± 4 K (Fig
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. This is in contrast with stabilization by kosmotropic buffers, which decrease the free energy of the native state
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. This apparent K^sub d^, which reflects protein-protein associations on a confined membrane surface, is predicted to be ~2 orders of magnitude weaker when the proteins are free to associate in solution (see Runnels and Scarlata, 1999)
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. In addition, apoA-2:DMPC disks were prepared by cholate dialysis (Jonas, 1986); EM, gel electrophoresis, and spectroscopic data of these disks and of the disks formed by spontaneous reconstitution were similar
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. To isolate the contribution of the helical junctions to tertiary folding, we also studied the corresponding ribozyme constructs without the loop on arm A (SL 2WJ, 3WJ, and 4WJ, Fig
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. Scores on the SOGS can range from 0 to 20
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