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Predissociated Dimers and Molten Globule Monomers in the Equilibrium Unfolding of Yeast Glutathione ReductaseLouzada, Paulo RobertoABSTRACTThe equilibrium unfolding of dimeric yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl) was investigated
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Native dimeric GR eluted at a volume corresponding to an apparent molecular weight of ~85 kDa in the calibrated column, indicating a fairly compact dimer structure
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The IR and fluorescence results strongly suggested that the thermal unfolding of ACYI could be illustrated as a two-stage process involving an intermediate: native state (N) [arrow right] intermediate stale (I) [arrow right] aggregates (A)
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Coming to the nature of the interactions, we see that both hydrogen bonding and hydrophobic interactions prevail in the system, the frequency of the latter occurring more often
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Of course, what we refer to as cavities will actually be filled by solvent and cosolule molecules under denaturing conditions
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Conformational Properties of Macromolecules
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and acquired every 3 K
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For instance, we would expect that the shortest life span would be found in cells directly interacting with the outside environment (eg, epithelial cells)
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On the other hand, the antigen recognized by Mab217 has not yet been characterized and could still act as an efferocytosis receptor, possibly through the recognition of PS
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At high temperatures, a steep increase in the light scattering is observed, suggesting an increase in the particle size and/or refractive index due to disk fusion
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Furthermore, the bi-exponential heat-unfolding kinetics observed in apoA-2: DMPC disks (Fig
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This is usually interpreted to mean that the residue in question enjoys the majority of its native contacts at the transition state (Fersht, 2000)
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