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This article deals with the unfolding reactions of the nonglycosylated recombinant form of the protein rSBA and its comparison with the glycosylated counterpart gSBA
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This result was also reported in a study of docking of EIC^sup tt^ with EIN^sup ec^ (11)
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As this is not available one can at best calculate the minimum average number of binding sites per macromolecule, n, by assuming the maximum fluorescence intensity increment, ~110-fold, ever observed between free and protein-bound ANS (φ^sub Fprotein^/φ^sub Fice^ = 0
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With each mutant azurin, a sizable fraction of binding sites exhibits affinities in the 1-2 mm range, corresponding to a dissociation constant 3-4 times lower than that of WT
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An important time window in the picosecond-to-nanosecond time range is occupied by atomic thermal fluctuations, which have been extensively investigated by molecular dynamics (MD) simulations (2), and by various experimental approaches including neutron scattering (3,4) and nuclear magnetic resonance (NMR) (5)
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75 ml g^sup -1^ (35) were assumed
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We produced one IPP and two EVA toners according to the optimized protocols discussed above
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Moreover, the Mg^sup 2+^-dependent docking efficiencies of 2WJ, 3WJ, and 4WJ ribozyme constructs determined by trFRET are generally in accord with the results shown in Fig
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Galera P, Redini F, Vivien D, et al
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Exp Cell Res 1992:200:379-92
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